Application of microcalorimeter energy measurement to biopolymer mass spectrometry

Author

Rabin, M.W. ; Hilton, G.C. ; Martinis, John M.

Author_Institution

Nat. Inst. of Stand. & Technol., Boulder, CO, USA

Volume

11

Issue

1

fYear

2001

fDate

3/1/2001 12:00:00 AM

Firstpage

242

Lastpage

247

Abstract

We have performed electrospray ionization mass spectrometry using a magnetic-sector mass spectrometer of proteins, detecting the ions with a normal-insulator-superconductor microcalorimeter detector. We emphasize the measurement of ion-impact energy as a way to obtain extra information that is unavailable in normal mass spectrometry. Energy measurements are used to discriminate against erroneous ion-strikes, to resolve ambiguities that cannot be resolved by normal mass spectrometry, and to illustrate some of the performance limits of the current detector design

Keywords

biological techniques; ion-surface impact; mass spectrometer accessories; molecular biophysics; particle calorimetry; proteins; superconducting particle detectors; biopolymer mass spectrometry; electrospray ionization mass spectrometry; energy measurements; erroneous ion-strikes; ion-impact energy; magnetic-sector mass spectrometer; microcalorimeter energy measurement; normal-insulator-superconductor microcalorimeter detector; performance limits; proteins; Current measurement; Detectors; Energy measurement; Energy resolution; Gain measurement; Ionization; Mass spectroscopy; Noise measurement; Proteins; Q measurement;

fLanguage

English

Journal_Title

Applied Superconductivity, IEEE Transactions on

Publisher

ieee

ISSN

1051-8223

Type

jour

DOI

10.1109/77.919329

Filename

919329