DocumentCode :
1655181
Title :
A High Frequency Vibrating System to Stimulate Cells in Bone Tissue Engineering
Author :
Prè, D. ; Magenes, G. ; Ceccarelli, G. ; De Angelis, M. G Cusella
Author_Institution :
Dept. of Inf. & Systemistic, Univ. of Pavia, Pavia
fYear :
2008
Firstpage :
884
Lastpage :
887
Abstract :
The aim of our work is to find a standard method to mechanically stimulate bone tissue progenitors cells with vibrations. We designed and realized a bioreactor in which we can change frequency, modality and duration of the stimuli. The system is composed by an eccentric motor, Voltage controlled, which can produce a displacement of 11 mm at frequencies between 1 Hz and 120 Hz, and by a plate connected to the motor allowing the contemporary vibration of three flasks or multiwells. We measured the acceleration with a 3D accelerometer to verify the accuracy and the repeatability of the stimuli imposed to the cells cultures. Consequently it has been possible to understand the relationship between these parameters and the effect on in vitro cells cultures. Particularly we focused on Saos-2 Human Osteoblasts to test the effects of high frequency vibrations on cells proliferation as a first pointer of the effects of the stimuli imposed by the bioreactor. We also realized the device for in vivo applications and we tested it on mice.
Keywords :
biomechanics; bioreactors; bone; cellular biophysics; tissue engineering; vibrations; 3D accelerometer; Saos-2 Human Osteoblasts; bioreactor; bone tissue engineering; cell stimulation; eccentric motor; high frequency vibrating system; Acceleration; Accelerometers; Bioreactors; Bone tissue; Control systems; Displacement control; Frequency; Testing; Vibration control; Voltage control;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Bioinformatics and Biomedical Engineering, 2008. ICBBE 2008. The 2nd International Conference on
Conference_Location :
Shanghai
Print_ISBN :
978-1-4244-1747-6
Electronic_ISBN :
978-1-4244-1748-3
Type :
conf
DOI :
10.1109/ICBBE.2008.217
Filename :
4535097
Link To Document :
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