The biocompatibility of the temperature sensitive pHsp-HSV-TK/As2O3 magnetic complex and its Anti-tumor Effect on HepG2 cells

We prepared PEI-As₂O₃/Mn_0.5Zn_0.5Fe₂O₄ magnetic complex as drug and pHsp-HSV-TK gene vector, at the same time it could induce the gene expressed by heat. Then the MTT assay, hemolysis test, the mouse of LD₅₀ test and micronucleus (MN) test were presented to evaluate the toxicity of it in vitro and in vivo . The therapeutic effects on cultured HepG2 cells treated by thermochemotherapy and pHsp70-HSV-TK of nanosized PEI-As₂O₃/Mn_0.5Zn_0.5Fe₂O₄ complex were evaluated by MTT test and flow cytometry. The experiments showed that the toxicity of the material on mouse fibroblast (L-929) cell lines was between 0~1 degree; It has no hemolysis activity; Its LD₅₀ arrived at 3.449 g/kg; Micronucleus test showed it has no genotoxic effects either. The therapeutic results in vitro showed that thermochemotherapy combined with gene therapy of PEI-As₂O₃/Mn_0.5Zn_0.5Fe₂O₄ complex could significantly inhibit cellular growth and obviously induce cellular apoptosis. Furthermore, the therapeutic results outweigh that of single As₂O₃ group and Mn_0.5Zn_0.5Fe₂O₄ group.