A resonance light scattering spectrometry study on the potential toxicity of xanthotoxin

Author

Qianqian Wang ; Junsheng Li ; Gao Yan ; Weilun Li ; Guoxia Huang ; Liujuan Yan

Author_Institution

Coll. of Biol. & Chem. Eng., Guangxi Univ. of Sci. & Technol., Liuzhou, China

fYear

2014

fDate

8-9 May 2014

Firstpage

775

Lastpage

777

Abstract

In this paper, the interaction between xanthotoxin and DNA was studied by resonance light scattering spectroscopy. Then the DNA saturation binding value with xanthotoxin was calculated and compared with the saturation values of ethidium bromide (EB) and bergapten. The saturation DNA binding value can be used as a basis to determine the intensity of drug molecule and DNA interactions. The greater the saturation value, the stronger the DNA intercalation ability and the potential toxicity of a given drug molecule. The results showed that the DNA saturation binding value of xanthotoxin is 0.18, and the saturation values for EB and bergapten are 14.70 and 0.18. As a consequence, it appears that the potential toxicity of xanthotoxin is lower than that of EB and equal with bergapten. The aim of this paper is to provide the basis and reference for using xanthotoxin and avoiding its possible risks.

Keywords

DNA; biochemistry; drugs; intercalation compounds; light scattering; molecular biophysics; molecular configurations; spectrochemical analysis; toxicology; DNA intercalation ability; DNA saturation binding value; bergapten; drug molecule intensity; ethidium bromide; potential toxicity; resonance light scattering spectrometry; saturation values; xanthotoxin-DNA interaction; DNA; DNA saturation binding value; Potential toxicity; Resonance light scattering; xanthotoxin;

fLanguage

English

Publisher

ieee

Conference_Titel

Electronics, Computer and Applications, 2014 IEEE Workshop on

Conference_Location

Ottawa, ON

Type

conf

DOI

10.1109/IWECA.2014.6845737

Filename

6845737