Automated cell counting method for microgroove based microfluidic device

Author

Khabiry, Masoud ; Jalili, Nader ; Sridhar, S.

fYear

2014

fDate

25-27 April 2014

Firstpage

1

Lastpage

2

Abstract

Microfluidic grooves and channels enable the control of cell positioning and capturing of cells in cell-based biosensor applications. Microfluidic devices also provide a platform for cell-based biological assays. Microfluidic channels have been used to capture cells for various cell-based diagnostics and screening applications. The number of cells and cell locations are important factors which will affect the flow pattern in the microfluidic device and consequently induced shear stresses. Moreover, manual cell counting and cell information extraction is a time consuming and tedious task. Automated techniques reduce human errors and expedite the process. Furthermore, it facilitates extraction of information from obtained images for further fluidic analysis.

Keywords

bioMEMS; biomedical equipment; cellular biophysics; image segmentation; medical image processing; microchannel flow; microsensors; shear flow; automated cell counting method; cell capture; cell information extraction; cell locations; cell positioning; cell-based biological assays; cell-based biosensor application; cell-based diagnostics; cell-based screening applications; flow pattern; microfluidic channels; microfluidic grooves; microgroove based microfluidic device; shear stresses; Data mining; Educational institutions; Image segmentation; Microfluidics; Microscopy; Stress; Automated Cell Counting; Cell Positioning; Microfluidics; Microgroove;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference (NEBEC), 2014 40th Annual Northeast

Conference_Location

Boston, MA

Type

conf

DOI

10.1109/NEBEC.2014.6972835

Filename

6972835