Title :
Visible brain-wide networks at single-neuron resolution with Micro-Optical Sectioning Tomography
Author_Institution :
Wuhan Nat. Lab. for Optoelectron., Huazhong Univ. of Sci. & Technol., Wuhan, China
Abstract :
We report a protocol combining a novel resin-embedding method for maintaining fluorescence, an automated fluorescence MOST system for long-term stable imaging, and a digital reconstruction-registration-annotation pipeline for tracing the axonal pathways individually in the mouse brain.
Keywords :
biomedical optical imaging; brain; fluorescence; image reconstruction; image registration; medical image processing; neurophysiology; optical tomography; resins; axonal pathways; digital reconstruction-registration-annotation pipeline; fluorescence MOST system; microoptical sectioning tomography; mouse brain; resin-embedding method; single-neuron resolution; visible brain-wide networks; Fluorescence; Mice; Microscopy; Spatial resolution; Tomography;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2014 Conference on
Conference_Location :
San Jose, CA
