Title :
Nonlinear fluorescence spectra unmixing
Author :
Ikoma, Hayato ; Heshmat, Barmak ; Wetzstein, Gordon ; Raskar, Ramesh
Author_Institution :
Media Lab., Massachusetts Inst. of Technol., Cambridge, MA, USA
Abstract :
We have developed a nonlinear spectral unmixing algorithm that separates fluorescence excitation-emission matrix of multiple fluorophores affected by the inner filter effect. We evaluate this technique on simulated data and demonstrate its superior performance experimentally for a mixture of fluorophores.
Keywords :
fluorescence; fluorescence spectroscopy; fluorescence excitation-emission matrix; inner filter effect; multiple fluorophores; nonlinear fluorescence spectra unmixing; nonlinear spectral unmixing algorithm; Absorption; Adaptive optics; Fluorescence; Nonlinear distortion; Optical distortion; Spectroscopy; Wavelength measurement;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2014 Conference on
Conference_Location :
San Jose, CA
