Title :
Bioinformatics analysis of the complete nucleotide sequence of htrA gene in Haemophilus parasuis
Author :
Luhua Zhang ; Yiping Wen ; Sanjie Cao ; Xintian Wen
Author_Institution :
Porcine Disease Res. Center, Sichuan Agric. Univ., Ya´an, China
Abstract :
A complete open reading frame of htrA gene and its encoding protein of Haemophilus parasuis were analyzed by multiple bioinformatics software. The htrA gene was composed of 1380 bp nucleotides encoding HtrA protein of 459 amino acids. The molecular weight of the protein was 48494.4 Da with the formula C2140H3505N597O665S8. The HtrA protein had the following characteristics: one signal peptide cleavage site, no transmembrane helix, located at periplasmic, numerous antigenic determinants, three conserved domains and a mixed type protein. Phylogenetic analysis showed that this protein in Haemophilus parasuis shared high homology with that in other reference species. All the results obtained in the present study will provide some basic information for further studies.
Keywords :
bioinformatics; diseases; encoding; evolution (biological); genetics; molecular biophysics; molecular configurations; molecular weight; proteins; Haemophilus parasuis; HtrA protein; amino acids; bioinformatic analysis; bioinformatics software; homology; htrA gene; molecular weight; nucleotide sequence; numerous antigenic protein; periplasmic protein; phylogenetic analysis; protein encoding; signal peptide cleavage site; Amino acids; Bioinformatics; Peptides; Phylogeny; Protein engineering; Proteins; Software; Haemophilus parasuis; bioinformatics; htrA gene;
Conference_Titel :
Biomedical Engineering and Informatics (BMEI), 2014 7th International Conference on
Conference_Location :
Dalian
Print_ISBN :
978-1-4799-5837-5
DOI :
10.1109/BMEI.2014.7002881
