Title :
Optical trapping of microspheres as a model for biological cells
Author :
Wright, W.H. ; Sonek, G.J. ; Berns, M.W.
Author_Institution :
California Univ., Irvine, CA, USA
Abstract :
An analytical model has been developed to predict, in three dimensions, the forces exerted on dielectric microspheres in an optical trap. Results for a single-beam gradient force trap predict the existence of a stable trapping location and an effective trapping range. A minimum trapping power of ~5 mW, and an effective axial range of ~9 μm, have been measured for polystyrene microspheres suspended in water and are in reasonable agreement with theoretical results. Applications of the model to the optical trapping of biological cells and chromosomes are described
Keywords :
biological techniques and instruments; cellular biophysics; radiation pressure; 5 mW; analytical model; biological cells; chromosomes; dielectric microspheres; effective axial range; effective trapping range; minimum trapping power; optical trap; polystyrene microspheres; single-beam gradient force trap; stable trapping location; water; Biological cells; Biological system modeling; Biomedical optical imaging; Charge carrier processes; Dielectrics; Laser beams; Optical beams; Optical scattering; Surface emitting lasers; Surface waves;
Conference_Titel :
Engineering in Medicine and Biology Society, 1989. Images of the Twenty-First Century., Proceedings of the Annual International Conference of the IEEE Engineering in
Conference_Location :
Seattle, WA
DOI :
10.1109/IEMBS.1989.96160
