Target DNA recognition using electrochemical impedance spectroscopy

Author

Michaels, Pauline ; Ciampi, Simone ; Yean, Chan Yean ; Gooding, J. Justin

Author_Institution

Sch. of Chem., Univ. of New South Wales, Sydney, NSW, Australia

fYear

2010

fDate

22-26 Feb. 2010

Firstpage

282

Lastpage

284

Abstract

Electrochemical impedance spectroscopy was used to differentiate between different target DNA sequences without the use of a redox label or application of large bias voltages. This method relies on the formation of a mixed self-assembled monolayer (SAMs) composed of single-stranded DNA and a diluent, mercaptoethanol. Recognition of the DNA analytes was achieved through the significant increase in conductance for the double stranded DNA, as compared to single-stranded DNA. Non-complementary and single base-pair mismatches were also able to be differentiated.

Keywords

DNA; biological techniques; electrochemical impedance spectroscopy; molecular biophysics; molecular configurations; monolayers; self-assembly; DNA analytes; DNA recognition; DNA sequences; double stranded DNA; electrochemical impedance spectroscopy; large bias voltages; mercaptoethanol; mixed self-assembled monolayer; redox label; single base-pair mismatches; single-stranded DNA; Biosensors; Capacitance; DNA; Electrochemical impedance spectroscopy; Electrodes; Gold; Probes; DNA; electrochemical impedance spectroscopy; gold-electrodes;

fLanguage

English

Publisher

ieee

Conference_Titel

Nanoscience and Nanotechnology (ICONN), 2010 International Conference on

Conference_Location

Sydney, NSW

Print_ISBN

978-1-4244-5261-3

Electronic_ISBN

978-1-4244-5262-0

Type

conf

DOI

10.1109/ICONN.2010.6045241

Filename

6045241