Title :
Polymer Based Microchip for Combined Capillary Electrophoresis and Electrochemical Detection
Author :
Rashid, M. ; Auger, V. ; Williams, L. ; Dou, Y. ; Pruneanu, S. ; Ali, Z.
Author_Institution :
Univ. of Teesside, Middlesbrough
Abstract :
We have developed Microsystems with capillary electrophoresis and an electrochemical detector (CE-ECD). The microfabricated CE-ECD systems can be used as a disposable device and the characteristics have been optimized for application in electrochemical detection. The system has been realized on a polystyrene (PS) polymer chip with gold electrodes. The injection and separation channels were produced by relatively simple and inexpensive methods. An electro-osmotic flow system (EOF) and a three-electrode electrochemical detector were fabricated on the same substrate in a single process. Cyclic voltammetry has been used to test the electrodes behaviour with catechol and dopamine in buffers of different pH. In this article, we give an overview on the methodological aspects of coupling ED (electrochemical detection) with capillary electrophoretic systems.
Keywords :
capillarity; electrochemical electrodes; electrochemical sensors; electrophoresis; osmosis; pH; polymers; voltammetry (chemical analysis); capillary electrophoresis; catechol; cyclic voltammetry; dopamine; electro-osmotic flow system; electrochemical detection; gold electrodes; polymer based microchip; polystyrene polymer chip; separation channels; three-electrode electrochemical detector; Chemical analysis; Chemical technology; Costs; Detectors; Electrodes; Electrokinetics; Fluorescence; Immune system; Polymers; Testing; Catechols; Dopamine; Electrochemistry; Electrodes; Electrophoresis, Capillary; Gold; Hydrogen-Ion Concentration; Microarray Analysis; Oxidation-Reduction; Platinum; Polymers;
Conference_Titel :
Engineering in Medicine and Biology Society, 2007. EMBS 2007. 29th Annual International Conference of the IEEE
Conference_Location :
Lyon
Print_ISBN :
978-1-4244-0787-3
DOI :
10.1109/IEMBS.2007.4352235