Title :
Heat shock induced apoptosis in normal and tumor human umbilical vein cells (HUVECs)
Author :
Mingjie Zhou ; Aili Zhang ; Xiaofang Hu ; Ping Liu ; Xu, L.X.
Author_Institution :
Shanghai Jiaotong Univ., Shanghai
Abstract :
Human endothelial cell injury may play an important role tumor damage hyperthermia in vivo. In this study, human umbilical vein cells were heat shocked in vitro at different temperatures (41-55degC). Cells were observed 0, 12, 24, 36, 48, 60 and 72 hours after heat shock. By incrementally increasing the heat load from 41-55degC, we observed a gradually increase in apoptosis with a significant change from apoptotic to necrotic death at temperature beyond 45degC. Further, an in vitro human breast tumor endothelial cell model was established to compare the heat shock response between normal and tumor endothelial cells.
Keywords :
blood vessels; cellular effects of radiation; hyperthermia; shock wave effects; tumours; apoptosis; breast tumor endothelial cell model; heat shock; human umbilical vein cells; hyperthermia; temperature 41 degC to 55 degC; time 12 hour; time 24 hour; time 36 hour; time 48 fs; time 72 hour; Breast tumors; Electric shock; Humans; Hyperthermia; In vitro; In vivo; Injuries; Neoplasms; Temperature; Veins; Apoptosis; Cell Line; Endothelial Cells; Heat-Shock Response; Hot Temperature; Humans; Vascular Neoplasms;
Conference_Titel :
Engineering in Medicine and Biology Society, 2007. EMBS 2007. 29th Annual International Conference of the IEEE
Conference_Location :
Lyon
Print_ISBN :
978-1-4244-0787-3
DOI :
10.1109/IEMBS.2007.4353097