Title :
Study of single-cell electroendocytosis using Bio-MEMS technology
Author :
Lin, Ran ; Chang, Donald C. ; Lee, Yi-Kuen
Author_Institution :
Bioeng. Grad. Program, Hong Kong Univ. of Sci. & Technol., Hong Kong, China
Abstract :
In this work, a micro electroendocytosis (EED) device was fabricated by MEMS technology to investigate the EED of mammalian cells. The corresponding endocytotic vesicles at the single-cell level were visualized using digital fluorescence video microscopy. The EED process under different electrical and biological parameters was studied in detail. The critical EED electric field strength for the HeLa cells using FM4-64 dye was determined to be 341.9plusmn24.2 V/cm. Beyond the critical electric field, the EED efficiency increased dramatically without significantly reducing cell viability. In addition, the EED efficiency as a function of multiple electric parameters were readily obtained based on large-scale micro EED experiments.
Keywords :
bioMEMS; bioelectric phenomena; biological effects of fields; biological techniques; cellular biophysics; fluorescence; optical microscopy; FM4-64 dye; HeLa cells; bioMEMS technology; critical EED electric field strength; digital fluorescence video microscopy; endocytotic vesicles; mammalian cell EED; micro-EED device; micro-electroendocytosis device; single cell electroendocytosis; Biomembranes; Fluorescence; Large-scale systems; Lipidomics; Micromechanical devices; Microscopy; Plasmas; Pulse generation; Radio access networks; USA Councils; Bio-MEMS; Electroendocytosis; FM4-64 dye; HeLa cell; electroporation; endocytotic vesicle;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285448
