Title :
A microfluidic device to control bio actuators of microorganisms, an application to Vorticella convallaria
Author :
Nagai, M. ; Ryu, S. ; Thorsen, T. ; Matsudaira, P. ; Fujita, H.
Author_Institution :
Inst. of Ind. Sci. (IIS), CIRMM, Univ. of Tokyo, Tokyo, Japan
Abstract :
We demonstrate a microfluidic device to control the motion of Vorticella convallaria by changing solution. Floating cells of V. convallaria were injected and cultured inside the device. Approximately 35 cells of V. convallaria in 102 nl of the solution. The cells were treated with saponin solution and controlled with a microfluidic device. When reaction solution was switched, the stalk contracted and relaxed corresponding to the ambient calcium ion concentration change. The length of the stalk changed between 40 and 60 mum, resulting in a working distance of about 20 mum.
Keywords :
bioMEMS; biochemistry; bioelectric phenomena; cellular biophysics; microactuators; microfluidics; microorganisms; Vorticella convallaria; bioactuator control; calcium ion concentration; cell culture; microfluidic device; microorganisms; size 40 mum to 60 mum; Actuators; Microfluidics; Microorganisms; Biomolecular motor; Microfluidic valve; Vorticella;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285468
