Title :
High throughput DNA methylation analysis on a droplet-in-oil polymerase chain reaction array
Author :
Zhang, Yi ; Bailey, Vasudev ; Puleo, Christopher M. ; Easwaran, Hariharan ; Griffiths, Elizabeth ; Herman, James G. ; Baylin, Stephen B. ; Wang, Tza-Huei
Author_Institution :
Dept. of Biomed. Eng., Johns Hopkins Univ., Baltimore, MD, USA
Abstract :
We performed on-chip DNA methylation analysis using methylation-specific PCR (MSP) within a high throughput microfluidic droplet array. The device uses of the oil phase as a companion fluid for both sample actuation and compartmentalization. These technical advantages allow for infusion of minute amounts of sample for arrayed MSP analysis, without the added complexities inherent in microfluidic droplet-based studies. Ease of use of this micro device is exemplified by analysis of two tumor suppressor promoters, p15 and TMS1 using an on-chip methylation assay. These results were consistent with standard MSP protocols, yet the simplicity of the droplet-in-oil microfluidic PCR platform provides an easy and efficient tool for DNA methylation analysis in a large-scale arrayed manner.
Keywords :
DNA; bioMEMS; biological specimen preparation; microfluidics; molecular biophysics; tumours; MSP analysis; TMS1 tumor suppressor promoter; droplet in oil PCR array; high throughput DNA methylation analysis; high throughput microfluidic droplet array; methylation specific PCR; on chip methylation assay; p15 tumor suppressor promoter; polymerase chain reaction; sample actuation; sample compartmentalisation; Cancer; DNA; Microchannel; Microfluidics; Minerals; Neoplasms; Petroleum; Polymers; Solvents; Throughput; DNA methylation; Droplet-in-oil; PCR;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285699
