Title :
Coherent anti-Stokes Raman scattering microscopy
Author :
Holtom, G.R. ; Zumbusch, A. ; Sunney Xie, X.
Author_Institution :
William R. Wiley Environ. Molecular Sci. Lab., Pacific Northwest Lab., Richland, WA, USA
Abstract :
Summary form only given. We report a new nonlinear optical method for high resolution microscopy which is based on a well established technique, coherent anti-Stokes Raman spectroscopy (CARS). The contrast mechanism for image formation depends on vibrational properties of the sample. This method is complementary to a variety of optical microscopies that have emerged recently, which use either linear or non-linear light interactions. Unlike confocal and two-photon fluorescence microscopy, native fluorescence or stain is not required, yet we share the capability of acquiring a stack of images to reconstruct three dimensional structure.
Keywords :
biological techniques; coherent antiStokes Raman scattering; optical images; optical microscopy; 3D structure reconstruction; CARS microscopy; contrast mechanism; four-wave mixing; high resolution microscopy; image formation; live cells; nonlinear optical method; polystyrene beads; sample vibrational properties; Frequency; Laser excitation; Laser tuning; Nonlinear optics; Optical microscopy; Optical mixing; Optical pumping; Optical scattering; Raman scattering; Ultrafast optics;
Conference_Titel :
Lasers and Electro-Optics, 1999. CLEO '99. Summaries of Papers Presented at the Conference on
Conference_Location :
Baltimore, MD, USA
Print_ISBN :
1-55752-595-1
DOI :
10.1109/CLEO.1999.833907
