Process flow for classification and clustering of fruit fly gene expression patterns

The rapidly growing collection of fruit fly embryo images makes automated Image Segmentation and classification an indispensable requirement for a large-scale analysis of in situ hybridization (ISH) - gene expression patterns (GEP). We present here such an automated process flow for segmenting, classification, and clustering large-scale sets of Drosophila melanogaster GEP that is capable of dealing with most of the complications implicated in the images.