Title :
Droplet flow focusing for molecular binding detection
Author :
Sunggu Kim ; Junghoon Lee
Author_Institution :
Seoul Nat. Univ., Seoul, South Korea
Abstract :
We report the detection of interfacial molecular binding via droplet generation in a flow focusing device. DNA hybridization at the phospholipid causes interfacial tension shift at the oil-water boundary which changes the mode of droplet production in the device. Droplet size decreased ~30% due to a complementary binding compared with non-complementary and control cases. Our report includes a molecular protocol to functionalize the interface with single-strand (ss) DNA as receptor and flow condition tuning for an unambiguous distinction of complementary binding.
Keywords :
DNA; bioMEMS; biosensors; drops; flow sensors; lipid bilayers; microfluidics; microsensors; molecular biophysics; molecular configurations; surface tension; DNA hybridization; complementary binding; droplet flow focusing device; droplet generation; droplet production mode; droplet size; flow condition; interfacial molecular binding detection; interfacial tension shift; molecular protocol; oil-water boundary; phospholipid; receptor; single-strand DNA; unambiguous distinction; DNA; Fluorescence; Focusing; Lipidomics; Microchannels; Microfluidics; Protocols;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2015 28th IEEE International Conference on
Conference_Location :
Estoril
DOI :
10.1109/MEMSYS.2015.7050929
