Title :
Infrared Light Induced Patterning of Proteins on ppNIPAM Thermoresponsive Thin Films: A “ Protein Laser Printer ”
Author :
Bohringer, K.F. ; Bilge, H. ; Xuanhong Cheng ; Ratner, B. ; Takeuchi, S. ; Fujita, H.
Author_Institution :
Department of Electrical Engineering, University of Washington, Seattle, WA, USA; Institute of Industrial Science, University of Tokyo, Komaba, Tokyo, JAPAN
Abstract :
Controlled adsorption and patterning of proteins on a thin film of thermo-responsive plasma-deposited poly-N-isopropylacrylamide occurs in response to heating with a computer-controlled infrared laser beam, with pattern resolution in the single micrometer range and adsorption times within a few seconds. Deposition of multiple proteins in the same substrate region has been accomplished. Successful streptavidin-biotin and antibody-antigen binding indicates that protein function stays intact after proteins adsorb to the ppNIPAM bio-polymer surface.
Keywords :
Infrared heating; Laser beams; Optical computing; Optical control; Particle beams; Plasmas; Proteins; Substrates; Temperature control; Transistors;
Conference_Titel :
Micro Electro Mechanical Systems, 2006. MEMS 2006 Istanbul. 19th IEEE International Conference on
Conference_Location :
Istanbul, Turkey
Print_ISBN :
0-7803-9475-5
DOI :
10.1109/MEMSYS.2006.1627853
