Title :
Human GITRLaa50-177 Expressed in Bac-to-Bac Baculovirus Expression System
Author :
Tang, Li ; Wang, Sheng-Jun ; Mao, Chao-Ming ; Chen, Jun ; Hu, Zheng-Jun ; Bao, Jun-Feng ; Shao, Qi-Xiang ; Xu, Hua-Xi
Author_Institution :
Dept. of Immunology, Jiangsu Univ., Jiangsu
Abstract :
Glucocorticoid induced tumor necrosis factor receptor ligand (GITRL) is a type II transmembrane protein with homology to TNF receptor superfamily members, and its human receptor (hGITR), an ortholog of the recently discovered murine glucocorticoid-induced TNFR-related (mGITR) protein. Ligation of murine GITR/GITRL can enhance immunity reactions via several signal pathways. However, so far the certain function of hGITR is not clear. In this study, we first used Bac-to-Bac baculovirus expression system to express hGITRLaa50-177 recombinant protein, then identified the recombinant protein by SDS-PAGE, western blot and indirect immunofluorescence staining.
Keywords :
biomembranes; cellular biophysics; fluorescence; microorganisms; molecular biophysics; proteins; tumours; Bac-to-Bac baculovirus expression system; TNF receptor superfamily members; glucocorticoid-induced TNFR-related protein; glucocorticoid-induced tumor necrosis factor receptor ligand; hGITRLaa50-177 recombinant protein; homology; human receptor; immunity reactions; indirect immunofluorescence staining; murine GITR-GITRL ligation; type-II transmembrane protein; Biological information theory; Biomedical engineering; Cells (biology); DNA; Extracellular; Humans; Ice; Immune system; Neoplasms; Proteins; GITRL; recombinant protein;
Conference_Titel :
BioMedical Engineering and Informatics, 2008. BMEI 2008. International Conference on
Conference_Location :
Sanya
Print_ISBN :
978-0-7695-3118-2
DOI :
10.1109/BMEI.2008.369
