Numerical modelling as a design optimisation tool for DNA electrophoresis

Author

Srivastava, A. ; Georghio, G.E. ; Metaxas, A.C. ; So, P. ; Matsudaira, P. ; Ehrlich, D.

Author_Institution

Whitehead Inst. for Biomed. Res., Cambridge, UK

fYear

2002

fDate

8-11 April 2002

Abstract

A two-dimensional finite-element flux-corrected transport algorithm developed for the solution of gas discharge problems is employed in the field of microfabricated electrophoretic devices used for DNA sequencing. This will assist in a comprehensive study of sample loading and injection in sample injectors in DNA electrophoresis and optimise system design. Results for the initial stages of loading and injection without pullback are introduced. Preliminary design optimisation investigates the adjustment of the pullback voltage for minimum DNA "leakage". The results demonstrate the capabilities of the numerical algorithm as a design and optimisation tool for DNA electrophoresis.

Keywords

DNA; biochemistry; biological techniques; electrophoresis; finite element analysis; molecular biophysics; DNA electrophoresis; DNA sequencing; design optimisation tool; microfabricated electrophoretic devices; minimum DNA leakage; numerical algorithm; numerical modelling; pullback voltage; sample injectors; sample loading; system design; two-dimensional finite-element flux-corrected transport algorithm;

fLanguage

English

Publisher

iet

Conference_Titel

Computation in Electromagnetics, 2002. CEM 2002. The Fourth International Conference on (Ref. No. 2002/063)

Type

conf

DOI

10.1049/ic:20020196

Filename

1225649