Title :
Using differential confocal microscopy to measure the membrane rigidity of a lipid vesicle in optical trap
Author :
Lee, Chau-Hwang ; Liu, Tze-Hsuan ; Xiao, Jian-Long ; Lin, Jiunn-Yuan
fDate :
Aug. 28 2011-Sept. 1 2011
Abstract :
We use laser tweezers to trap a lipid vesicle and differential confocal microscopy to measure the out-of-plane fluctuations of the membrane. Bending rigidities of fluidic- and gel-phase membranes are obtained from the measured fluctuation amplitudes.
Keywords :
bending; biomechanics; lipid bilayers; mechanical variables measurement; optical microscopy; radiation pressure; shear modulus; differential confocal microscopy; fluidic-phase membranes; gel-phase membranes; laser tweezers; lipid vesicle; optical trap; out-of-plane fluctuations; rigidity; Biomembranes; Fluctuations; Lipidomics; Measurement by laser beam; Microscopy; Optical filters; Optical variables measurement;
Conference_Titel :
Quantum Electronics Conference & Lasers and Electro-Optics (CLEO/IQEC/PACIFIC RIM), 2011
Conference_Location :
Sydney, NSW
Print_ISBN :
978-1-4577-1939-4
DOI :
10.1109/IQEC-CLEO.2011.6193648
