Title :
Riptide: fast protein identification from mass spectrometer data
Author :
Carter, Richard J.
Author_Institution :
Computational Biosci. Res., Adv. Studies HP Lab., Palo Alto, CA, USA
Abstract :
The biotechfirm target discovery incorporated (TDI) has developed a relatively fast and inexpensive method for protein identification. The final step in their approach involves an algorithm to deduce the terminal amino acid sequence of an unknown intact protein from its fragmentation mass spectrum. TDI´s web-published algorithm was taken as a starting point for further research. The algorithm Riptide was developed that matches the output of TDI´s algorithm, but demonstrates a 193X speed improvement on a 6-deep sequencing.
Keywords :
mass spectroscopy; molecular biophysics; proteins; sequential estimation; Riptide; biotechfirm; fast protein identification; fragmentation mass spectrum; mass spectrometer; target discovery incorporated; terminal amino acid sequence; web-published algorithm; Amino acids; Art; Chemicals; Databases; Impedance matching; Laboratories; Mass spectroscopy; Peptides; Protein engineering; Sequences;
Conference_Titel :
Bioinformatics Conference, 2003. CSB 2003. Proceedings of the 2003 IEEE
Print_ISBN :
0-7695-2000-6
DOI :
10.1109/CSB.2003.1227344