A comparison of deglycerolization processes

Author

Reddy, H. ; Draheim, J. ; Rudolph, D. ; Coughlin, R.

Author_Institution

SymBiotech. Inc., Wallingford, CT, USA

fYear

2000

fDate

2000

Firstpage

115

Lastpage

116

Abstract

This study compared a standard centrifugation based method for deglycerolizing cryopreserved RBCs and a proprietary, automated method based upon hollow fiber membrane (HFM) filtration. The methods were compared by examining the characteristics (i.e. glycerol concentration, quantity of RBC and hemoglobin, osmotic fragility) of the product of each. Deglycerolization with the filtration method yielded products with final glycerol concentrations less than 1% w/v, as did the centrifugation. Deglycerolized RBC units from each process exhibited no significant difference in the osmotic fragility or in the quantity of RBCs and hemoglobin available (99% level)

Keywords

biological techniques; blood; cellular biophysics; filtration; freezing; osmosis; automated method; cell freezing; cryopreserved red blood cells; deglycerolization processes; final glycerol concentration; glycerol concentration; hemoglobin quantity; hollow fiber membrane filtration; long-term storage; osmotic fragility; standard centrifugation based method; sterility; Assembly; Cells (biology); Extracellular; Filtration; Gravity; Ice; Laboratories; Protocols; Red blood cells; Testing;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference, 2000. Proceedings of the IEEE 26th Annual Northeast

Conference_Location

Storrs, CT

Print_ISBN

0-7803-6341-8

Type

conf

DOI

10.1109/NEBC.2000.842406

Filename

842406