Acoustoelectronic method for the detection of electrical and mechanical properties of adhering cell cultures

Author

Guhr, G. ; Brünig, R. ; Schmidt, H. ; Weihnacht, M.

Author_Institution

Leibniz Inst. of Solid State & Mater. Res. Dresden, Dresden, Germany

fYear

2009

fDate

20-23 Sept. 2009

Firstpage

677

Lastpage

680

Abstract

In this paper a new tool to assess viscoelastic and dielectric properties of biological samples is presented. Shear horizontal polarized surface acoustic waves (SH-SAW) are used to detect the viscoelastic properties of biological samples. Therefore a one-port SAW resonator, with a fundamental mode of 85 MHz was developed. Its electrode structure can be used simultaneously to detect the dielectric behavior of the whole system by impedance spectroscopy (IS) while the frequency ranges from kHz to MHz. The applicability of the combination of both methods appearing as new tool is exemplarily shown by cell adhesion experiments performed with murine fibroblast cell line L929.

Keywords

bioelectric phenomena; biological techniques; biomechanics; cellular biophysics; dielectric properties; elastic waves; surface acoustic wave resonators; surface acoustic waves; ultrasonic measurement; viscoelasticity; L929 murine fibroblast cell line; SAW resonator electrode structure; acoustoelectronic method; adhering cell cultures; cell adhesion experiments; cell electrical properties; cell mechanical properties; dielectric properties; electrical property detection; frequency 85 MHz; impedance spectroscopy; mechanical property detection; one port SAW resonator; polarised SH-SAW; shear horizontal surface acoustic waves; viscoelastic properties; Acoustic signal detection; Acoustic waves; Dielectrics; Elasticity; Electrodes; Mechanical factors; Polarization; Surface acoustic waves; Surface impedance; Viscosity; L929; cell adhesion process; cell based biosensor; impedance spectroscopy; surface acoustic wave;

fLanguage

English

Publisher

ieee

Conference_Titel

Ultrasonics Symposium (IUS), 2009 IEEE International

Conference_Location

Rome

ISSN

1948-5719

Print_ISBN

978-1-4244-4389-5

Electronic_ISBN

1948-5719

Type

conf

DOI

10.1109/ULTSYM.2009.5441737

Filename

5441737