Development of a Simultaneously Time- and Spectrum-Resolved Multifocal Multiphoton Microscopy System Using a Streak Camera

Author

Liu, L. ; Qu, J. ; Wang, L. ; Fu, Z. ; Lin, Z. ; Guo, B. ; Niu, H.

Author_Institution

Key Lab. of Optoelectron. Devices & Syst. of Minist. of Educ., Shenzhen Univ., Shenzhen

fYear

2006

fDate

16-18 Oct. 2006

Firstpage

186

Lastpage

189

Abstract

We report the development of a multifocal multiphoton microscope (MMM) system using a specially designed streak camera, which is capable of providing simultaneously time- and spectrum-resolved fluorescence microscopy. Temporal and spectral resolutions of the system have been calibrated to be 6.5-200 ps and 1-3 nm respectively. We demonstrate the applicability of the system to spectrum-resolved fluorescence and lifetime imaging with fluorescent dyes, fluorescent microspheres. Potential applications of the system include the measurement of tissue pathology, intracellular physiology etc.

Keywords

biomedical optical imaging; dyes; fluorescence; medical image processing; optical microscopy; streak cameras; fluorescent dyes; fluorescent microspheres; intracellular physiology; lifetime imaging; multifocal multiphoton microscope system; simultaneously time-spectrum resolved multifocal multiphoton microscopy system; spectrum-resolved fluorescence; streak camera; tissue pathology measurement; Biomedical measurements; Cameras; Fluorescence; High-resolution imaging; Image resolution; Optical imaging; Optical microscopy; Optical pulses; Spatial resolution; Ultrafast optics;

fLanguage

English

Publisher

ieee

Conference_Titel

Biophotonics, Nanophotonics and Metamaterials, 2006. Metamaterials 2006. International Symposium on

Conference_Location

Hangzhou

Print_ISBN

0-7803-9773-8

Electronic_ISBN

0-7803-9774-6

Type

conf

DOI

10.1109/METAMAT.2006.335031

Filename

4134770