Title :
Time-resolved Two-photon Excitation Fluorescence Spectroscopy Using a Streak Camera
Author :
Liu, Lixin ; Qu, Junle ; Lin, Ziyang ; Guo, Baoping ; Liu, Wenqing ; Niu, Hanben
Author_Institution :
Shenzhen Univ., Shenzhen
Abstract :
We present a time-resolved two-photon excitation fluorescence spectroscopy system that is based on a picosecond streak camera and a prism spectrophotometer, and can perform simultaneous fluorescence spectrum and lifetime measurement using two-photon excitation. The potential applications of the system in biomedicine have been demonstrated with tissue endogenous fluorophore (flavin adenine dinucleotide), plant specimen (aloe epidermis) and gastric smooth muscle tissue.
Keywords :
biomedical optical imaging; fluorescence spectroscopy; muscle; optical prisms; spectrophotometers; streak cameras; time resolved spectroscopy; two-photon processes; two-photon spectroscopy; aloe epidermis; biomedicine; flavin adenine dinucleotide; gastric smooth muscle tissue; plant specimen; prism spectrophotometer; streak camera; time-resolved spectroscopy; tissue endogenous fluorophore; two-photon excitation fluorescence spectroscopy; Biomedical engineering; Biomedical imaging; Biomedical measurements; Cameras; Charge-coupled image sensors; Epidermis; Fluorescence; Microscopy; Muscles; Spectroscopy;
Conference_Titel :
Complex Medical Engineering, 2007. CME 2007. IEEE/ICME International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-1077-4
Electronic_ISBN :
978-1-4244-1078-1
DOI :
10.1109/ICCME.2007.4381883
