Title :
Artificial tissue bioreactor (ATB) for biological and imaging applications
Author :
Whitehead, T.D. ; Nemanich, S.T. ; Shoghi, K.I.
Author_Institution :
Div. of Radiol. Sci., Mallinckrodt Inst. of Radiol. (MIR), St. Louis, MO, USA
fDate :
Aug. 28 2012-Sept. 1 2012
Abstract :
Three-dimensional (3D) culture systems are increasingly applied to study tissue biology. In this work, we report on the development of an artificial tissue bioreactor (ATB) designed to simulate the 3D structure and microenvironment of tissues in vivo, with multiple avenues of sampling, including the tissue chamber, for downstream analysis. Additionally, the ATB is integrated with the microPET Focus F220 for in-vivo imaging applications. As a proof-of-concept, we characterized the effects of lipids on glucose utilization using HepG2 cells. ATB studies were performed pre- and post- therapeutic intervention with the PPAR-γ agonist pioglitazone. In parallel, Glucose Tolerance Test (GTT) is performed on media samples to assess glucose uptake by cells as a measures of insulin signaling sensitivity. Fatty acid uptake in the ATB cell chamber is measured using [11C]Palmitate with microPET imaging. Overall, the ATB will facilitate the use of existing and novel radiopharmaceuticals in discovery of validating and translating insights derived from ATB studies to pre-clinical animal studies, to clinical evaluation.
Keywords :
biological techniques; biological tissues; bioreactors; cellular biophysics; positron emission tomography; 3D culture systems; ATB; GTT; HepG2 cells; PPAR-gamma agonist pioglitazone; artificial tissue bioreactor; biological applications; glucose tolerance test; glucose utilization; in vivo imaging applications; in vivo tissue 3D microenvironment; in vivo tissue 3D structure; lipids; microPET Focus F220; post therapeutic intervention; pretherapeutic intervention; radiopharmaceuticals; tissue biology; tissue chamber; Effluents; Feeds; Lipidomics; Media; Positron emission tomography; Sugar; Animals; Artificial Organs; Biomimetics; Bioreactors; Diagnostic Imaging; Ecosystem; Equipment Design; Equipment Failure Analysis; Humans; Organ Culture Techniques;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2012 Annual International Conference of the IEEE
Conference_Location :
San Diego, CA
Print_ISBN :
978-1-4244-4119-8
Electronic_ISBN :
1557-170X
DOI :
10.1109/EMBC.2012.6346452