A novel rapid-reaction nucleic-acid amplification device using micro-volume chips

In the study, we construct a novel rapid-reaction nucleic-acid amplification device and micro-volume chips utilizing semiconductor microfabrication technology. The temperature variation rate of the novel nucleic-acid amplification device achieves 2°C per second and it greatly decreases reaction time. Moreover, we develop the micro-volume chips fabricated by silicon wafer with two concentric circles structure for the nucleic-acid amplification. Comparing to the traditional method, the nucleic-acid amplification device and micro-volume chips have the advantages of extremely low-volume reaction solution (1~2 μ L) and high reaction efficiency (less than 30 minutes for full reaction). According to this research results, the novel rapid-reaction device and micro-volume chips are quite promising to efficiently nucleic-acid amplify for healthcare and rapid disease diagnoses.