DocumentCode :
2111047
Title :
An approach towards bronchoscopic-based gene therapy using electrical field accelerated plasmid droplets
Author :
Hradetzky, D. ; Boehringer, S. ; Geiser, Th ; Gazdhar, A.
Author_Institution :
Sch. of Life Sci., Univ. of Appl. Sci. & Arts Northwestern Switzerland, Muttenz, Switzerland
fYear :
2012
fDate :
Aug. 28 2012-Sept. 1 2012
Firstpage :
5753
Lastpage :
5756
Abstract :
Idiopathic pulmonary fibrosis (IPF) is a devastating disease affecting the distal lung, due to failure of the alveolar epithelium to heal after micro-injuries, leading to inefficient gas exchange and resulting in death. Therapeutic options are very limited. A new therapeutic approach based on gene therapy restores the self-healing process within the lung in the experimental setup. A basic requirement of this therapy is the successful transduction of genes into the alveolar epithelium in the distal part of the lung, for which a new therapeutic instrument is required. In this paper we present the concept and first experimental results of a device which uses an electrical field to accelerate the charged droplets of plasmid suspension toward the tissue and which overcomes cell membrane with its impact energy. The aim is to develop a therapeutic device capable of being integrated into minimally invasive procedures such as bronchoscopy.
Keywords :
bioelectric phenomena; biological tissues; biomedical equipment; biomembranes; cellular biophysics; drops; gene therapy; molecular biophysics; suspensions; alveolar epithelium; biological tissue; bronchoscopic-based gene therapy; bronchoscopy; cell membrane; charged droplet; field accelerated plasmid droplet; gene transduction; minimally invasive procedure; plasmid suspension; therapeutic device; Acceleration; Educational institutions; Electrodes; Fluids; Fluorescence; Gene therapy; Lungs; Animals; Bronchoscopy; Cells, Cultured; Electricity; Genetic Therapy; Idiopathic Pulmonary Fibrosis; Microscopy, Fluorescence; Plasmids; Rats;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2012 Annual International Conference of the IEEE
Conference_Location :
San Diego, CA
ISSN :
1557-170X
Print_ISBN :
978-1-4244-4119-8
Electronic_ISBN :
1557-170X
Type :
conf
DOI :
10.1109/EMBC.2012.6347301
Filename :
6347301
Link To Document :
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