3D engineered neural networks coupled to Micro-Electrode based devices: a new experimental model for neurophysiological applications

To study the electrophysiological activity and dynamics of a large neuronal population, Micro-Electrode Array (MEA) based systems are routinely used. 3-D culture models are nowadays considered a way to better mimic the in vivo situation. Here we present a simple approach based on the concept that dissociated cultured neurons are able to grow on micrometric, self-assembled microbeads to form a structurally and functionally connected 3D networks. With this model, we show preliminary results that indicate the possible use of such new experimental system for neuropharmacological studies.