Title :
Generation of large-area tunable uniform electric fields in microfluidic arrays for rapid DNA separation
Author :
Huang, L.R. ; Tegenfeldt, J.O. ; Kraeft, J.J. ; Sturm, James C. ; Austin, R.H. ; Cox, E.C.
Author_Institution :
Dept. of Electr. Eng., Princeton Univ., NJ, USA
Abstract :
Control of electric fields over large areas is crucial for the accurate delivery and manipulation of biologically important molecules in microfluidic systems. In this paper we present a novel method for generating tunable uniform electric fields over large microfluidic arrays in two dimensions, and its application to a microfabricated device that separates genomic DNA. The device fractionates large DNA molecules over three orders of magnitude faster than conventional methods.
Keywords :
DNA; arrays; bioelectric phenomena; biological techniques; electric fields; electrophoresis; microfluidics; separation; biologically important molecule delivery; biologically important molecule manipulation; electrical current injection method; electrophoresis; fractionation; genomic DNA; large-area tunable uniform electric field generation; microfabricated device; microfluidic arrays; rapid DNA separation; Bioinformatics; Biological materials; Boundary conditions; Current; DNA; Electric potential; Electrodes; Electrokinetics; Genomics; Microfluidics;
Conference_Titel :
Electron Devices Meeting, 2001. IEDM '01. Technical Digest. International
Conference_Location :
Washington, DC, USA
Print_ISBN :
0-7803-7050-3
DOI :
10.1109/IEDM.2001.979512
