Title :
Ultra-fast 4D microscopy and high throughput distributed deconvolution
Author :
Sibarita, J.-B. ; Magnin, H. ; De Mey, J.R.
Abstract :
Ultra-fast tri-dimensional time lapse (4D) imaging can record structures labelled by fluorescence-tagged proteins moving in all directions at speeds reaching 2-3 μm/sec. It has proven potential for describing hitherto hidden aspects of cellular processes and for studying protein function, but the approach is still in its childhood. Here, we detail a novel 4D acquisition system and the adaptation of a constrained iterative deconvolution algorithm, not widely used in light microscopy, which yields greatly improved image quality at much increased speed on a standard PC. Nevertheless, the amount of data generated by ultra-fast 4D-imaging, typically 2 to 3 Gb/hour in many thousands of film requires a lot of time to be processed off line. We present a solution based on network distribution, using available computers connected together in LAN, which we believe to be a first step towards online deconvolution.
Keywords :
biological techniques; cellular biophysics; deconvolution; fluorescence; image restoration; iterative methods; optical information processing; optical microscopy; proteins; 4D acquisition system; LAN; cellular processes; constrained iterative deconvolution algorithm; fluorescence-tagged proteins; high throughput distributed deconvolution; image quality; light microscopy; network distribution; off line processing; online deconvolution; protein function; standard PC; ultra-fast 4D microscopy; ultra-fast tri-dimensional time lapse imaging; Computer networks; Deconvolution; Distributed computing; Fluorescence; Image quality; Iterative algorithms; Local area networks; Microscopy; Proteins; Throughput;
Conference_Titel :
Biomedical Imaging, 2002. Proceedings. 2002 IEEE International Symposium on
Print_ISBN :
0-7803-7584-X
DOI :
10.1109/ISBI.2002.1029371
