Detection of Alcohol with Vertically Aligned Carbon Nanofiber (VACNF)

A reagentless amperometric enzymatic biosensor is constructed using Vertically Aligned Carbon Nanofiber (VACNF) for the detection of alcohol. Yeast alcohol dehydrogenase (YADH), and its cofactor nicotinamide adenine dinuleotide (NAD⁺) are immobilized by covalent attachment and adsorption to the carbon substrate. Electrochemical techniques are employed to test the function and performance of the constructed biosensor. Characterization of the electrode is performed using NADH. Subsequently, amperometric measurements are conducted with the electrodes for the detection of ethanol to determine the response of the electrical current due to an increase in analyte concentration. The detection range, storage stability, reusability, and response time of the biosensor is also examined.