Title :
Study of the combined effects of X-rays and low-energy microwave electromagnetic field on the state of chromatin in the nuclei of human buccal epithelium cells
Author :
Pasyuga, V.N. ; Mavrodi, S.A. ; Kuznetsov, K.A. ; Badian, E.E. ; Shurinov, R.V. ; Ivanchenko, D.D. ; Shckorbatov, Y.G.
Author_Institution :
V.N. Karazin Kharkiv Nat. Univ., Kharkov, Ukraine
Abstract :
Isolated human buccal epithelium cells of two men (22 and 25 years) and women (15 years) were irradiated with X-rays (200 and 800 μRh/h) for 1 minute, and microwaves (frequency - 36.6 GHz, the power density at the object level - 100 μW/cm2) during 1 min. X-rays were used before and after microwave irradiation, with interval in 20 min. The number of granules of heterochromatin was determined after orcein staining immediately after irradiation. It was shown that the response of cells to radiation differs in some way for cells of different donors. Common features in the reaction of cells - the number of heterochromatin granules after irradiation at 200 μRh/h was significantly higher than after irradiation intensity of 800 μRh/h. Microwave irradiation before and after X-ray treatment virtually eliminates these differences.
Keywords :
DNA; biological effects of X-rays; biological effects of fields; biological effects of microwaves; biomolecular effects of radiation; cellular effects of radiation; X-ray effects; X-ray irradiation; frequency 36.6 GHz; heterochromatin granules; isolated human buccal epithelium cell nuclei; low-energy microwave electromagnetic field effects; microwave irradiation; orcein staining; time 1 min; time 20 min; Electronic mail; Humans; Ionizing radiation; Masers; Radiation effects; Stress; X-rays;
Conference_Titel :
Microwave and Telecommunication Technology (CriMiCo), 2011 21th International Crimean Conference
Conference_Location :
Sevastopol
Print_ISBN :
978-1-4577-0883-1