FRET detection in the plasma membrane using Total Internal Reflection Fluorescence Lifetime Imaging Microscopy

Author

Blandin, P. ; Leveque-Fort, S. ; Lecart, S. ; Druon, F. ; Georges, P. ; Cossec, J.C. ; Potier, M.C. ; Lenkei, Z.

Author_Institution

Lab. Charles Fabry de l´´Inst. d´´Opt., Univ. Paris-Sud, Palaiseau

fYear

2008

fDate

4-9 May 2008

Firstpage

1

Lastpage

2

Abstract

We developed a total internal reflection fluorescence lifetime imaging microscope to perform functional imaging of living cells membranes labeled with FRET couples. Forster resonance energy transfer efficiency can thus be followed with subwavelength axial resolution.

Keywords

biological techniques; biomembranes; cellular biophysics; fluorescence; laser beam applications; optical microscopy; radiative lifetimes; FRET detection; Forster resonance energy transfer; biological processes; fluorophores; functional imaging; living cell membrane; picosecond supercontinuum fibre laser; plasma membrane; subwavelength axial resolution; total internal reflection fluorescence lifetime imaging microscopy; Biomedical measurements; Biomedical optical imaging; Biomembranes; Fluorescence; Image resolution; Microscopy; Optical filters; Optical imaging; Optical reflection; Plasmas; (170.6920) Time resolved imaging; (180.2520) Fluorescence microscopy;

fLanguage

English

Publisher

iet

Conference_Titel

Lasers and Electro-Optics, 2008 and 2008 Conference on Quantum Electronics and Laser Science. CLEO/QELS 2008. Conference on

Conference_Location

San Jose, CA

Print_ISBN

978-1-55752-859-9

Type

conf

Filename

4571311