Title :
DNA kinetics in microfabricated devices
Author :
Yick Chuen Chan ; Ma, R.M.S. ; Carles, M. ; Sucher, N.J. ; Man Wong ; Zohar, Y.
Author_Institution :
Dept. of Mech. Eng., Hong Kong Univ. of Sci. & Technol., Kowloon, China
Abstract :
The DNA kinetics in micro-capillary electrophoresis is presented. The mobility and diffusion coefficient of 14bp-DNA fragments as a function of concentration in two types of separation sieving matrices, hydroxyethylcellulose (HEC) polymer solution and agarose gel, are extracted through a series of experiments performed in microfabricated devices. In addition, the motion of a DNA plug through a miter bend and splitting a plug in a branch are quantitatively characterized. The concept of equivalent length is introduced to quantify the effect of a bend on the DNA plug motion. In a branching system, a simple kinematic relationship was discovered relating the quantity of DNA in each downstream branch to its relative channel cross-sectional area.
Keywords :
DNA; biochemistry; biodiffusion; biological techniques; capillarity; electrochemical analysis; electrophoresis; molecular biophysics; 14bp-DNA fragment mobility; DNA kinetics; DNA plug motion; agarose gel; branching system; channel cross-sectional area; diffusion coefficient; downstream branch; equivalent length; hydroxyethylcellulose polymer solution; kinematic relationship; micro-capillary electrophoresis; microfabricated devices; miter bend; separation sieving matrices; Bonding; DNA; Electrodes; Electrokinetics; Glass; Kinetic theory; Packaging; Plugs; Polymer gels; Sputter etching;
Conference_Titel :
Micro Electro Mechanical Systems, 2002. The Fifteenth IEEE International Conference on
Conference_Location :
Las Vegas, NV, USA
Print_ISBN :
0-7803-7185-2
DOI :
10.1109/MEMSYS.2002.984083
