Title :
Phase-contrast Microscopy by In-line Phase-shifting Digital Holography
Author :
Kang, Jeon Woong ; Hong, Chung Ki
Author_Institution :
Dept. of Phys., Pohang Univ. of Sci. & Technol., Pohang
Abstract :
Recently we have developed a precise quantitative phase-contrast microscope by in-line phase- shifting digital holography. The phase resolution of the system is 1.30 with blank image and which is better than those obtained with off-axis systems. The three-dimensional shape of a living fibroblast cell is measured with this system.
Keywords :
biological techniques; cellular biophysics; holography; optical microscopes; 3D shape; living fibroblast cell; phase resolution; phase-contrast microscopy; phase-shifting digital holography; Cells (biology); Fibroblasts; Focusing; Holographic optical components; Holography; Image reconstruction; Optical microscopy; Optical refraction; Optical variables control; Shape measurement;
Conference_Titel :
Lasers and Electro-Optics - Pacific Rim, 2007. CLEO/Pacific Rim 2007. Conference on
Conference_Location :
Seoul
Print_ISBN :
978-1-4244-1173-3
Electronic_ISBN :
978-1-4244-1174-0
DOI :
10.1109/CLEOPR.2007.4391691
