Title :
Non-invasive, optical monitoring and characterization of biochemical changes during stem cell differentiation
Author :
Quinn, K.P. ; Georgakoudi, I.
Author_Institution :
Dept. of Biomed. Eng., Tufts Univ., Medford, MA, USA
Abstract :
Multi-photon excited fluorescence microscopy was utilized to monitor the biochemical changes associated with the development of silk-based engineered constructs into functional adipose tissue. Based on the intrinsic fluorescence of NADH and FAD, cells within vascularized adipose tissue demonstrated significantly higher metabolic activity than cells in non-vascularized control tissue.
Keywords :
bio-optics; biochemistry; cellular biophysics; fluorescence; optical microscopy; tissue engineering; biochemical change; functional adipose tissue; intrinsic fluorescence; multiphoton excited fluorescence microscopy; noninvasive monitoring; optical monitoring; silk-based engineered constructs; stem cell differentiation; Fluorescence; Imaging; In vitro; Monitoring; Pixel; Stem cells; Three dimensional displays;
Conference_Titel :
Information Photonics (IP), 2011 ICO International Conference on
Conference_Location :
Ottawa, ON
Print_ISBN :
978-1-61284-315-5
DOI :
10.1109/ICO-IP.2011.5953743
