Title :
A point-of-care diagnostic prototype for high-throughput, multiplexed single-virus detection
Author :
Reddington, Alexander ; Trueb, Jacob T. ; Daaboul, George G. ; Freedman, David S. ; Fawcett, H.E. ; Connor, John H. ; Unlu, M.S.
Author_Institution :
Dept. of Electr. & Comput. Eng., Boston Univ., Boston, MA, USA
Abstract :
Attention to viral infections has increased after multiple recent outbreaks: the global 2009 H1N1 influenza pandemic, the variant-strain Ebola virus epidemic of 2007, and the West Nile virus epidemic of 2003-2004. Despite advances in antiviral drugs, diagnostics have lagged behind [1] [2]. These outbreaks underline the growing need for early virus discovery through rapid and reliable point-of-care (POC) diagnostics. POC diagnostics are limited to symptomatic patients as they rely on antibody-antigen interactions for detection. Plaque titer and real-time PCR are the standard methods for pre-seroconversion detection of an infection. Currently, these methods are laborious and do not lend well to POC applications. The Interferometric Reflectance Imaging Sensor (IRIS) is a portable, label-free, and multiplexed method for pre-seroconversion detection of single viral particles.
Keywords :
cellular biophysics; drugs; microorganisms; patient diagnosis; West Nile virus epidemic; antibody-antigen interactions; antiviral drugs; early virus discovery; global 2009 H1N1 influenza pandemic; high-throughput single-virus detection; interferometric reflectance imaging sensor; multiplexed single-virus detection; plaque titer; point-of-care diagnostic prototype; pre-seroconversion detection; real-time PCR; reliable point-of-care diagnostics; symptomatic patients; variant-strain Ebola virus epidemic; viral infections; Iris; Multiplexing;
Conference_Titel :
Photonics Conference (IPC), 2012 IEEE
Conference_Location :
Burlingame, CA
Print_ISBN :
978-1-4577-0731-5
DOI :
10.1109/IPCon.2012.6358532
