Point-of-care Parvovirus B19 detection and genotyping based on microfluidics and chemiluminescence “contact” imaging detection

Author

Dolci, Luisa Stella ; Mirasoli, Mara ; Buragina, Angela ; Guardigli, Massimo ; Roda, Aldo ; Bonvicini, Francesca ; Furio, Francesca Di

Author_Institution

Dept. of Pharm. Sci., Univ. of Bologna, Bologna, Italy

fYear

2011

fDate

8-10 June 2011

Firstpage

1

Lastpage

3

Abstract

A diagnostic device suitable for Parvovirus B19 genotyping and quantitative DNA detection was developed, based on hybridization with immobilized oligonucleotide probes and chemiluminescence detection. The microfluidic chamber was directly coupled with a CCD sensor in a “contact” imaging configuration to obtain a portable device suitable for point-of-care applications.

Keywords

CCD image sensors; DNA; bioMEMS; biomedical optical imaging; biosensors; chemiluminescence; microfluidics; microorganisms; molecular biophysics; CCD sensor; chemiluminescence contact imaging detection; chemiluminescence detection; diagnostic device; hybridization; immobilized oligonucleotide probes; microfluidic chamber; point of care Parvovirus B19 detection; point of care Parvovirus B19 genotyping; portable device; quantitative DNA detection; Calibration; Charge coupled devices; Glass; Imaging; Microfluidics; Performance evaluation; Probes;

fLanguage

English

Publisher

ieee

Conference_Titel

BioPhotonics, 2011 International Workshop on

Conference_Location

Parma

Print_ISBN

978-1-4244-9836-9

Electronic_ISBN

978-1-4244-9835-2

Type

conf

DOI

10.1109/IWBP.2011.5954864

Filename

5954864