Title :
Silicon micro-ring resonators for label-free aptamer-based biosensing
Author :
Park, Mi Kyoung ; Yiying, Jessie Quah ; Kee, Jack Sheng ; Song, Junfeng ; Kao, Linus Tzu-Hsiang ; Qing, Fang ; Lo, Guo-Qiang ; Netto, Vivian ; Fosse, Eric Mouchel La
Author_Institution :
Inst. of Microelectron., A*STAR (Agency for Sci., Technol. & Res.), Singapore, Singapore
Abstract :
We present a label-free aptamer sensor based on an array of silicon micro-ring resonators. An array of sensors consists of three sensor rings and a reference ring was fabricated and its surface sensitivity and the limit of detection was obtained using polyelectrolyte multilayer. The mass sensitivity and the limit of detection of the fabricated sensor ring is 0.35 nm/ng mm-2 and 42.8 pg/mm2 in air and 0.24 nm/ngmm-2 and 62.5 pg/mm2 in water, respectively. We successfully demonstrated the binding assay over a broad range of protein concentrations using a model system with human thrombin as the analyte and DNA aptamers as immobilized ligands.
Keywords :
DNA; bioMEMS; biochemistry; biosensors; elemental semiconductors; enzymes; microcavities; microfabrication; micromechanical resonators; microsensors; molecular biophysics; sensitivity; silicon; DNA aptamers; Si; binding assay; human thrombin; immobilized ligands; label-free aptamer sensor array; label-free aptamer-based biosensing; mass sensitivity; polyelectrolyte multilayer; protein concentrations; silicon microring resonators; surface sensitivity; Arrays; Biosensors; Optical surface waves; Optical waveguides; Sensitivity; Silicon; Surface waves; Aptamer-based sensor; Biophotonic Sensor; Silicon Micro-ring Resonator; Thrombin detection;
Conference_Titel :
Industrial Electronics and Applications (ICIEA), 2012 7th IEEE Conference on
Conference_Location :
Singapore
Print_ISBN :
978-1-4577-2118-2
DOI :
10.1109/ICIEA.2012.6360980