1I-5 Key Factors that Affect Sonoporation Efficiency in In Vitro Settings

Ultrasound induced intracellular drug delivery, sonoporation, is an appealing and promising technique for next generation drug delivery system. Many types of molecules, such as plasmid DNAs, siRNAs and peptides, have been demonstrated to be delivered into the cell by ultrasound with the aid of microbubbles both in vitro and in vivo. Although there are many reports on in vitro sonoporation, the efficiencies of successful sonoporation and the viabilities of cells after the procedure documented in each report varies in a wide range, and the reasons for these differences are not fully understood. In this study, we have investigated how different experimental settings would affect sonoporation efficiencies and cell viabilities after the procedure