Physical properties and biocompatibility of acemannan scaffold for biomedical applications

The aim of this study is to synthesize the biocompatible acemannan scaffold for biomedical application by chemical-crosslinked method. Adipic acid and CDI were employed as a crosslinker and a coupling agent, respectively. The three dimensional porous structure was constructed by the salt leaching method and then with freeze-dried process. Crross-linking analysis and surface morphology were examined by infrared spectroscopy and scanning electron microscope, respectively. Swelling rate and In Vitro degradation were determined by the ratio of weight changes. For biocompatibility test, the specimens were incubated with osteoprogenitor cell line, ST2. Cell viability was tested by biochemical assay of Presto Blue™. The result shows that the amount of adipic acid affected on the swelling rate and in vitro degradation. Biochemical assay demonstrated that acemannan scaffold exhibited biocompatibility by incubated with ST2 cells. These data suggest that adipic acid could be used as a crosslinker which affected on physical properties of acemannan scaffold. Moreover, the synthesized sponges have biocompatibility in In vitro level as well as can potentially be used in biomedical application.