Plasmonic nanohole arrays for label-free kinetic biosensing in a lipid membrane environment

Author

Lesuffleur, Antoine ; Lim, Kwan Seop ; Lindquist, Nathan C. ; Im, Hyungsoon ; Warrington, Arthur E. ; Rodriguez, Moses ; Oh, Sang-Hyun

Author_Institution

Dept. of Electr. & Comput. Eng., Univ. of Minnesota, Minneapolis, MN, USA

fYear

2009

fDate

3-6 Sept. 2009

Firstpage

1481

Lastpage

1484

Abstract

We integrate periodic nanohole arrays in a thin gold film with lipid membranes in a microfluidic channel. Surface plasmon-enhanced light transmission through the periodic nanohole arrays enables real-time label-free sensing of molecular binding on the lipid membrane surface. This membrane biosensor can potentially act as a natural platform for studying binding kinetics of proteins with their binding partners anchored in the lipid membrane. We also present the concept of using nanopore arrays for kinetic assays of transmembrane proteins in a free-standing lipid membrane.

Keywords

bio-optics; biomembrane transport; biosensors; gold; lipid bilayers; microfluidics; microsensors; molecular biophysics; nanobiotechnology; nanosensors; optical sensors; plasmonics; proteins; surface plasmons; thin film sensors; Au; free-standing lipid membrane environment; integrated periodic nanohole arrays; label-free kinetic biosensing; lipid membrane biosensor; microfluidic channel; molecular binding; plasmonic nanohole arrays; proteins binding kinetics; real-time label-free sensing; surface plasmon-enhanced light transmission; thin gold film; transmembrane protein kinetic assays; Biosensing Techniques; Kinetics; Membrane Lipids; Microscopy, Electron, Scanning; Surface Plasmon Resonance;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE

Conference_Location

Minneapolis, MN

ISSN

1557-170X

Print_ISBN

978-1-4244-3296-7

Electronic_ISBN

1557-170X

Type

conf

DOI

10.1109/IEMBS.2009.5332429

Filename

5332429