Title :
Multi-color raman nanotags for tumor cell biomarker detection
Author :
Nyagilo, James ; Xiao, Ming ; Sun, Xiankai ; Davé, Digant P.
Author_Institution :
Bioeng. Dept., Univ. of Texas at Arlington, Arlington, TX, USA
Abstract :
We report the synthesis and characterization of multi-color gold nanoparticle based tags (Raman nanotags) for molecular imaging. The multi-color Raman nanotags are PEGylated gold nanoparticles (AuNPs) encapsulating a Raman reporter dyes which can be functionalized with any ligand of interest for targeted molecular imaging. The Raman nanotags synthesized with 65 nm gold nanoparticles exhibit the largest surface enhanced Raman scattering (SERS) signal. Results are presented quantify the measured SERS signal, dynamic range, reproducibility, and stability of Raman nanotags. In vitro cell culture experiments for targeted biomarker detection using functionalized Raman nanotags are also presented.
Keywords :
biomedical materials; cellular biophysics; gold; molecular biophysics; nanobiotechnology; nanoparticles; surface enhanced Raman scattering; tumours; Au; PEGylated gold nanoparticle; SERS; biomarker; cell culture; molecular imaging; multicolor Raman nanotags; size 65 nm; surface enhanced Raman scattering; tumor cell; Brain Neoplasms; Cell Line, Tumor; Colorimetry; Contrast Media; Humans; Nanostructures; Particle Size; Spectrum Analysis, Raman; Staining and Labeling;
Conference_Titel :
Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE
Conference_Location :
Minneapolis, MN
Print_ISBN :
978-1-4244-3296-7
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2009.5332780
