Filamentous Phage-Based Extra Cellular Matrix

Filamentous bacteriophage (fd or M13) is commonly used in phage display experiments. In addition, the phage has recently been used in the bio-nanotechnology and biomaterial fields. To explore the potentials of filamentous bacteriophage as materials, we started to create a novel phage-based extra cellular matrix (ECM). As the first step, we prepared a titanium plate that was decorated with the TBP-1-displaying phage, which has the ability to bind to the surface of titanium, and investigated its biocompatibility. We also prepared a hydrogel of the filamentous phage by crosslinking phages with glutaraldehyde, and tested its in vitro biocompatibility using the murine cell line.