DocumentCode :
2422102
Title :
Study on the Gelsolin Effects in Oral Epithelia Carcinoma (OEC) Cells by Atomic Force Microscopy and Micro-pillars Arrays
Author :
Chen, Wei-Ren ; Huang, Pei-Yu ; Wang, Chien-Wen ; Lin, Yi-Dong ; Lin, Chi-Chang ; Chang, Hsien-Chang
Author_Institution :
Inst. of Biomed. Eng., Nat. Cheng Kung Univ.
fYear :
2007
fDate :
16-19 Jan. 2007
Firstpage :
1129
Lastpage :
1132
Abstract :
According to the concept of cell physiology, when the cells contact the extracellular matrix (ECM), the cell will adhere, spread and migrates. Despite the sophistication of experimental and computational approaches in cell and molecular biology, the mechanisms by which cells sense and respond to mechanical stimuli are poorly understood. In order to evaluate the characteristics of the cellular physiology, we use atomic force microscopy (AFM) and micro-pillars arrays (MPAs) to measure the cell mechanics of the oral epithelia carcinoma cells (OEC-M1). After AFM measurement, the stiffness of the OEC-Ml-wt was 41-95 kPa, meanwhile the OEC-M1-g6 was 21-46 kPa. In deed, 0.88-4.75 nN of the traction force was generated in OEC-M1-g6 cells, which the actin filament assemble around the cytoplasm might caused by the gelsolin, after forward, both the adherent and moving ability of the cells were increased.
Keywords :
atomic force microscopy; cancer; cellular biophysics; Gelsolin effects; atomic force microscopy; cell physiology; extracellular matrix; micro pillars arrays; oral epithelia carcinoma cells; Atomic force microscopy; Atomic measurements; Biology computing; Cells (biology); Computational biology; Electrochemical machining; Extracellular; Force measurement; Mechanical variables measurement; Physiology; AFM; ECM; MPAs; OEC cell; migration;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Nano/Micro Engineered and Molecular Systems, 2007. NEMS '07. 2nd IEEE International Conference on
Conference_Location :
Bangkok
Print_ISBN :
1-4244-0610-2
Type :
conf
DOI :
10.1109/NEMS.2007.352216
Filename :
4160519
Link To Document :
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