Influence of high-power EM-videopulse on insulated cells

The influence of a short high-power electromagnetic videopulse on a suspension of hepatic cells of 9-weeks human embryo has been investigated. Such pulse was generated by an electron beam of the accelerator "Temp-A" with energy 0.6 MeV, current 4 kA, and time duration 40 nsec falling to rod antenna of 3 m length. The output power was 100 MW. The samples were placed perpendicular to the antenna at 30 cm distance from its center. The free-radical state in the system "hepatocite+hypochlorite" has been investigated using the quantum-metrical set with photo-electronic amplifier FEU-140 in a thermostat box at 37/spl deg/C. The appearance of radical fragments in this system was registered by means of 630 nm spectral line of generated single oxygen. The estimation of the cell viability before and after irradiation was performed using the method of supravital staining by methylene dark blue. It was shown that the influence of video-pulse treatment decreases the percentage of the viability of the hepatocites and activates the free-radical processes and correspondingly enhances the single oxygen generation. The dependence of these processes on the video-pulse power was determined.