Effects of Chrysanthemum morifolium on expressions of p53 and Bax in the Human Umbilical Vein Cells under Induction and Cultivation of high glucose

Author

Liu, Yinghui ; Mou, Xin

Author_Institution

Endocrinology Dept., Hangzhou Red Cross Hosp., Hangzhou, China

fYear

2011

fDate

24-26 June 2011

Firstpage

6795

Lastpage

6798

Abstract

Objective : To discuss the effects of Chrysan them um morifolium on mRNA expressions of p53 and Bax in the Human Umbilical Vein Cells(HUVEC) under Induction and Cultivation of high glucose. Methods: Make four groups of the HUVEC as normal cells group, normal glucose only deal cells group high glucose -only deal cells group and Chrysanthemum morifolium + high glucose acting cells group. After 18~24h, the mRNA expressions of p53 and Bax were detected by reverse transcription-polymerase chain reaction (RT-PCR) method. Results: High glucose can up-regulation the mRNA expressions of p53 and Bax, and Chrysanthemum morifolium can decline the mRNA expressions of p53 and Bax in a dose-dependent manner,and the role of middle-dose is most manifest. Conclusion: Chrysanthemum morifolium can down-regulation the mRNA expressions of p53 and Bax, to prevent and cure diabetic vascular complications.

Keywords

biochemistry; blood vessels; cellular biophysics; enzymes; molecular biophysics; sugar; Bax expression; Chrysanthemum morifolium; HUVEC; diabetic vascular complication; glucose acting cell group; human umbilical vein cells; mRNA expression; p53 expression; reverse transcription-polymerase chain reaction method; Hospitals; Humans; RNA; Roads; Stress; Sugar; Veins; Bax; Chrysanthemum morifolium; high glucose; p53; vascular endothelial cells;

fLanguage

English

Publisher

ieee

Conference_Titel

Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on

Conference_Location

Nanjing

Print_ISBN

978-1-4244-9172-8

Type

conf

DOI

10.1109/RSETE.2011.5965924

Filename

5965924